H226 cells

Disease:

Squamous cell carcinoma; mesothelioma

Origin:

This cell line was derived from metastatic site: pleural effusion in March of 1980 from a patient with squamous cell carcinoma of the lung

Species:

Homo sapiens

Tissue:

Lung, Pleural effusion

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

modal number = 47; range = 32-88; del(p25)

Patient:

Male

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS

Subculture:

When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO