H1650 cells

Disease:

Stage 3B, adenocarcinoma; bronchoalveolar carcinoma

Origin:

The H1650 cell line was derived from the metastatic site of pleural effusion from a smoker.

Species:

Homo sapiens, human

Tissue:

Lung

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Not specified

Patient:

Male, Caucasian, 27 years of age smoker.

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS

Subculture:

Split sub-confluent cultures (70-80%) at a seed ratio of 1:4 to 1:6. i.e. seeding at 5x10, 000 cells/cm^2 using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO