WERI-Rb-1 cells

Disease:
Retinoblastoma
Origin:
This cell line was isolated by explant culture of a primary tumor from the right eye obtained immediately after enucleation in 1974.
Species:
Homo sapiens
Tissue:
Eye, retina
Morphology:
Grape-like clusters of round cells
Properties:
Suspension
Cytogenic data:
This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1, ?), t(3p, 5q), der(3)t(?q29; ?), t(3q, ?), 5q+, i(6p), t(7q, ?), 9q+, t(10q, 21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations
Patient:
Female, 1 yrs of age, Caucasian
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
Subculture:
Seed at 2.5x10^5 cells/mL, maintain at 1.0 x 106 cells/ml; feed cells twice-weekly. Culture at 5% CO2; 37C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative

Cell Lines