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Retinoblastoma
This cell line was isolated by explant culture of a primary tumor from the right eye obtained immediately after enucleation in 1974.
Homo sapiens Tissue:Eye, retina Morphology:Grape-like clusters of round cells Properties: Suspension Cytogenic data: This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1, ?), t(3p, 5q), der(3)t(?q29; ?), t(3q, ?), 5q+, i(6p), t(7q, ?), 9q+, t(10q, 21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations Patient: Female, 1 yrs of age, Caucasian Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:Seed at 2.5x10^5 cells/mL, maintain at 1.0 x 106 cells/ml; feed cells twice-weekly. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Eye, retina Morphology:Grape-like clusters of round cells Properties: Suspension Cytogenic data: This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1, ?), t(3p, 5q), der(3)t(?q29; ?), t(3q, ?), 5q+, i(6p), t(7q, ?), 9q+, t(10q, 21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations Patient: Female, 1 yrs of age, Caucasian Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:Seed at 2.5x10^5 cells/mL, maintain at 1.0 x 106 cells/ml; feed cells twice-weekly. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Grape-like clusters of round cells Properties: Suspension Cytogenic data: This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1, ?), t(3p, 5q), der(3)t(?q29; ?), t(3q, ?), 5q+, i(6p), t(7q, ?), 9q+, t(10q, 21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations Patient: Female, 1 yrs of age, Caucasian Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:Seed at 2.5x10^5 cells/mL, maintain at 1.0 x 106 cells/ml; feed cells twice-weekly. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Suspension Cytogenic data: This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1, ?), t(3p, 5q), der(3)t(?q29; ?), t(3q, ?), 5q+, i(6p), t(7q, ?), 9q+, t(10q, 21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations Patient: Female, 1 yrs of age, Caucasian Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:Seed at 2.5x10^5 cells/mL, maintain at 1.0 x 106 cells/ml; feed cells twice-weekly. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1, ?), t(3p, 5q), der(3)t(?q29; ?), t(3q, ?), 5q+, i(6p), t(7q, ?), 9q+, t(10q, 21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations Patient: Female, 1 yrs of age, Caucasian Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:Seed at 2.5x10^5 cells/mL, maintain at 1.0 x 106 cells/ml; feed cells twice-weekly. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Female, 1 yrs of age, Caucasian Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:Seed at 2.5x10^5 cells/mL, maintain at 1.0 x 106 cells/ml; feed cells twice-weekly. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS Subculture:Seed at 2.5x10^5 cells/mL, maintain at 1.0 x 106 cells/ml; feed cells twice-weekly. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Seed at 2.5x10^5 cells/mL, maintain at 1.0 x 106 cells/ml; feed cells twice-weekly. Culture at 5% CO2; 37C Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Complete culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Hepatitis B: Negative Hepatitis C: Negative
Hepatitis C: Negative
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
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