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Nodular lymphoma
Mouse x Human hybrid myeloma. Derived by a fusion of P3/NSI/1-Ag4-1 myeloma cells with cells from a human nodular lymphoma. The cells are HPRT- and do not secrete immunoglobulin. They are used as a fusion partner for human B lymphocytes. The cells are deficient in hypoxanthine phosphoribosyltransferase (HPRT) and are excellent fusion partners for human B cells. Tested and found negative for ectromelia virus (mousepox).
Human (B cell lymphoma)/mouse (myeloma) hybrid Tissue: Heterohybridoma, hybridoma Morphology: Lymphoblast Properties: Suspension Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
Heterohybridoma, hybridoma Morphology: Lymphoblast Properties: Suspension Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
Lymphoblast Properties: Suspension Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
Suspension Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
Complete culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
If use of this culture results in a scientific publication, it should be cited in the publication as: K6H6/B5 (AddexBio C0033002)
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