K6H6/B5 cells

Disease:

Nodular lymphoma

Origin:

Mouse x Human hybrid myeloma. Derived by a fusion of P3/NSI/1-Ag4-1 myeloma cells with cells from a human nodular lymphoma. The cells are HPRT- and do not secrete immunoglobulin. They are used as a fusion partner for human B lymphocytes. The cells are deficient in hypoxanthine phosphoribosyltransferase (HPRT) and are excellent fusion partners for human B cells. Tested and found negative for ectromelia virus (mousepox).

Species:

Human (B cell lymphoma)/mouse (myeloma) hybrid

Tissue:

Heterohybridoma, hybridoma

Morphology:

Lymphoblast

Properties:

Suspension

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium + 10% FBS

Subculture:

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/ml and maintain between 1 X 10^5 and 1 X 10^6 cells/ml. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO