FHs 74 Int

Disease:

Normal

Origin:

This cell line was isolated from human normal epithelial tissues by collagenase digestion and selective removal of fibroblasts with trypsin-Versene.

Species:

Homo sapiens, human

Tissue:

Small intestine

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Normal female; diploid

Patient:

Female, 3-4 months gestation

Medium:

AddexBio-formulated DMEM (C0003-01) + Insulin (10 ug/mL) + 10% FBS (Do not filter the medium after addition of insulin)

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO