FHs 74 Int

Disease:
Normal
Origin:
This cell line was isolated from human normal epithelial tissues by collagenase digestion and selective removal of fibroblasts with trypsin-Versene.
Species:
Homo sapiens, human
Tissue:
Small intestine
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Normal female; diploid
Patient:
Female, 3-4 months gestation
Medium:
AddexBio-formulated DMEM (C0003-01) + Insulin (10 ug/mL) + 10% FBS (Do not filter the medium after addition of insulin)
Subculture:
Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 10, 11
D13S317: 11, 12
D16S539: 11, 12
D5S818: 11, 12
D7S820: 9, 10
THO1:7, 9.3
TPOX: 8
vWA: 16, 18
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)

Cell Lines