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Normal
This cell line was isolated from human normal epithelial tissues by collagenase digestion and selective removal of fibroblasts with trypsin-Versene.
Homo sapiens, human Tissue: Small intestine Morphology: Epithelial Properties: Adherent Cytogenic data: Normal female; diploid Patient: Female, 3-4 months gestation Medium: AddexBio-formulated DMEM (C0003-01) + Insulin (10 ug/mL) + 10% FBS (Do not filter the medium after addition of insulin) Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Small intestine Morphology: Epithelial Properties: Adherent Cytogenic data: Normal female; diploid Patient: Female, 3-4 months gestation Medium: AddexBio-formulated DMEM (C0003-01) + Insulin (10 ug/mL) + 10% FBS (Do not filter the medium after addition of insulin) Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Epithelial Properties: Adherent Cytogenic data: Normal female; diploid Patient: Female, 3-4 months gestation Medium: AddexBio-formulated DMEM (C0003-01) + Insulin (10 ug/mL) + 10% FBS (Do not filter the medium after addition of insulin) Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Adherent Cytogenic data: Normal female; diploid Patient: Female, 3-4 months gestation Medium: AddexBio-formulated DMEM (C0003-01) + Insulin (10 ug/mL) + 10% FBS (Do not filter the medium after addition of insulin) Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Normal female; diploid Patient: Female, 3-4 months gestation Medium: AddexBio-formulated DMEM (C0003-01) + Insulin (10 ug/mL) + 10% FBS (Do not filter the medium after addition of insulin) Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Female, 3-4 months gestation Medium: AddexBio-formulated DMEM (C0003-01) + Insulin (10 ug/mL) + 10% FBS (Do not filter the medium after addition of insulin) Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
AddexBio-formulated DMEM (C0003-01) + Insulin (10 ug/mL) + 10% FBS (Do not filter the medium after addition of insulin) Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Hepatitis B: Negative DNA Profile (STR): Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Amelogenin: X CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
CSF1PO: 10, 11 D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
D13S317: 11, 12 D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
D16S539: 11, 12 D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
D5S818: 11, 12 D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
D7S820: 9, 10 THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
THO1:7, 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
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