Comparison

FHs 74 Int

Item no. P0021002
Manufacturer Addexbio
Amount One Frozen vial
Category
Type Tumour Cells
Specific against Human (Homo sapiens)
Dry ice Yes
Citations 1. Owens RB, Smith HS, Nelson-Rees WA, Springer EL. Epithelial cell cultures from normal and cancerous human tissues. J Natl Cancer Inst. 1976;56(4):843-849. PMID: 176412
ECLASS 10.1 32160890
ECLASS 11.0 32160890
UNSPSC 41106509
Available
Description

Disease:

Normal

Origin:

This cell line was isolated from human normal epithelial tissues by collagenase digestion and selective removal of fibroblasts with trypsin-Versene.

Species:

Homo sapiens, human

Tissue:

Small intestine

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Normal female; diploid

Patient:

Female, 3-4 months gestation

Medium:

AddexBio-formulated DMEM (C0003-01) + Insulin (10 ug/mL) + 10% FBS (Do not filter the medium after addition of insulin)

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO

Biosafety Level:

I

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

HIV: Negative

Hepatitis B: Negative

DNA Profile (STR):

Amelogenin: X

CSF1PO: 10, 11

D13S317: 11, 12

D16S539: 11, 12

D5S818: 11, 12

D7S820: 9, 10

THO1:7, 9.3

TPOX: 8

vWA: 16, 18

Citation Format:

If use of this culture results in a scientific publication, it should be cited in the publication as: FHs 74 Int (AddexBio P0021002)

Storage condition
LN2
Manufacturers Category
Cell Lines

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: One Frozen vial
Available: In stock
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