HK-2 cells

Disease:
Papilloma
Origin:
HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney..
The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes.
The cell line appears to be derived from a single cell based on Southern and FISH analysis.
Species:
Human, Homo sapiens
Tissue:
Kidney, cortex/proximal tubule
Morphology:
Epithelial
Properties:
Adherent
Patient:
Male, adult
Medium:
Keratinocyte Growth Medium (serum-free)(C00013-34)
Subculture:
Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin/EDTA. Culture at 5% CO2, 37C. Feed cells once every 2 to 3 days.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
II
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 13
D13S317: 9
D16S539: 11, 12
D5S818: 12
D7S820: 10, 11
THO1: 9
TPOX: 8, 9
vWA: 17, 18

Cell Lines