THLE-3 cells

Disease:
Normal, SV40 immortalized, Non-tumorigenic in athymic nude mice.
Origin:
THLE-3 cell line was derived from primary normal liver cells by infection with SV40 large T antigen. The cells express cytokeratin 18 and albumin in early passage, whereas higher-passage cells in logarithmic-phase growth also express cytokeratin 19.
Species:
Homo sapiens
Tissue:
Liver, left lobe
Morphology:
Epithelial;
Properties:
Adherent
Cytogenic data:
Near diploid
Patient:
Adult
Medium:
BEGM + additives without anti-fungal reagents
Subculture:
When confluent using 0.05% trypsin or trypsin/EDTA, subculture at 1:2 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Flask pre-coating information please refer to datasheet.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
II
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
DNA Profile (STR):
Amelogenin: X
CSF1PO: 11, 12
D13S317: 13
D16S539: 11, 12
D5S818: 13
D7S820: 8, 10
THO1: 8, 9.3
TPOX: 6, 9
vWA: 17, 18

Cell Lines