THLE-3 cells

Disease:

Normal, SV40 immortalized, Non-tumorigenic in athymic nude mice.

Origin:

THLE-3 cell line was derived from primary normal liver cells by infection with SV40 large T antigen. The cells express cytokeratin 18 and albumin in early passage, whereas higher-passage cells in logarithmic-phase growth also express cytokeratin 19.

Species:

Homo sapiens

Tissue:

Liver, left lobe

Morphology:

Epithelial;

Properties:

Adherent

Cytogenic data:

Near diploid

Patient:

Adult

Medium:

BEGM + additives without anti-fungal reagents

Subculture:

When confluent using 0.05% trypsin or trypsin/EDTA, subculture at 1:2 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Flask pre-coating information please refer to datasheet.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO