Origin:
BSC40 is a high-temperature tolerant derivative of BS-C-1 established by Brockman and Nathans. BSC40 cells may be grown at temperature from 31 to 40C. The cells are useful for selection and assay of temperature sensitive viruses. They support the growth of vaccinia virus, and can be used as a host for vaccinia virus genetic studies.
Species:
Cercopithecus aethiops, African Green Monkey Tissue:
Kidney Morphology:
Epithelia Properties:
Adherent Host:
African Green Monkey Medium:
DMEM Medium + 10% FBS Subculture:
When reaching 80%, subculture using 0.25% trypsin or trypsin/EDTA at 1:6 to 1:10; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I Sterility:
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens:
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative If use of this culture results in a scientific publication, it should be cited in the publication as: BSC40 (AddexBio T0011003)