BSC40 cells

Origin:

BSC40 is a high-temperature tolerant derivative of BS-C-1 established by Brockman and Nathans. BSC40 cells may be grown at temperature from 31 to 40C. The cells are useful for selection and assay of temperature sensitive viruses. They support the growth of vaccinia virus, and can be used as a host for vaccinia virus genetic studies.

Species:

Cercopithecus aethiops, African Green Monkey

Tissue:

Kidney

Morphology:

Epithelia

Properties:

Adherent

Host:

African Green Monkey

Medium:

DMEM Medium + 10% FBS

Subculture:

When reaching 80%, subculture using 0.25% trypsin or trypsin/EDTA at 1:6 to 1:10; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO