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Myelogenous leukemia
The M-NFS-60 cell line was derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirus. The cells are responsive to both interleukin 3 (interleukin-3, IL-3) and macrophage colony stimulating factor (M-CSF). The cells contain a truncated c-myb proto - oncogene caused by integration of a retrovirus. This cell line is a suitable transfection host.
Mus musculus, mouse Morphology: Lymphoblast Properties: Suspension Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium 2-mercaptoethanol (0.05 mM) + human recombinant macrophage colony stimulating factor (M-CSF)(70 ng/mL) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5 X 10^4 viable cells/mL. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)
Lymphoblast Properties: Suspension Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium 2-mercaptoethanol (0.05 mM) + human recombinant macrophage colony stimulating factor (M-CSF)(70 ng/mL) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5 X 10^4 viable cells/mL. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)
Suspension Medium: AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium 2-mercaptoethanol (0.05 mM) + human recombinant macrophage colony stimulating factor (M-CSF)(70 ng/mL) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5 X 10^4 viable cells/mL. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)
AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium 2-mercaptoethanol (0.05 mM) + human recombinant macrophage colony stimulating factor (M-CSF)(70 ng/mL) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5 X 10^4 viable cells/mL. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5 X 10^4 viable cells/mL. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)
Complete culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)
Hepatitis B: Negative Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)
Hepatitis C: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)
If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)
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