M-NFS-60 cells

Disease:

Myelogenous leukemia

Origin:

The M-NFS-60 cell line was derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirus. The cells are responsive to both interleukin 3 (interleukin-3, IL-3) and macrophage colony stimulating factor (M-CSF). The cells contain a truncated c-myb proto - oncogene caused by integration of a retrovirus. This cell line is a suitable transfection host.

Species:

Mus musculus, mouse

Morphology:

Lymphoblast

Properties:

Suspension

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium 2-mercaptoethanol (0.05 mM) + human recombinant macrophage colony stimulating factor (M-CSF)(70 ng/mL) + 10% FBS

Subculture:

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5 X 10^4 viable cells/mL. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO