M-NFS-60 cells

Disease:
Myelogenous leukemia
Origin:
The M-NFS-60 cell line was derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirus. The cells are responsive to both interleukin 3 (interleukin-3, IL-3) and macrophage colony stimulating factor (M-CSF). The cells contain a truncated c-myb proto - oncogene caused by integration of a retrovirus. This cell line is a suitable transfection host.
Species:
Mus musculus, mouse
Morphology:
Lymphoblast
Properties:
Suspension
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) , or equivalent medium 2-mercaptoethanol (0.05 mM) + human recombinant macrophage colony stimulating factor (M-CSF)(70 ng/mL) + 10% FBS
Subculture:
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2.5 X 10^4 viable cells/mL. Culture at 5% CO2, 37C.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: M-NFS-60 (AddexBio C0003022)

Cell Lines