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Animals were immunized with W/Fu rat thymocytes. Spleen cells were fused with NS-1 myeloma cells.
Mouse, Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) Tissue:hybridoma: B lymphocyte Morphology: Lymphoblast Properties: Suspension Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture:Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 10^5 viable cells/mL. Maintain cultures at a cell concentration between 1 x 10^5 and 1 x 10^6cells/mL. Do not allow the cell concentration to exceed 1 x 10^6cells/mL. Culture at 5% CO2, 37C. Feed cells once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 50% FBS and 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
hybridoma: B lymphocyte Morphology: Lymphoblast Properties: Suspension Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture:Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 10^5 viable cells/mL. Maintain cultures at a cell concentration between 1 x 10^5 and 1 x 10^6cells/mL. Do not allow the cell concentration to exceed 1 x 10^6cells/mL. Culture at 5% CO2, 37C. Feed cells once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 50% FBS and 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Lymphoblast Properties: Suspension Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture:Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 10^5 viable cells/mL. Maintain cultures at a cell concentration between 1 x 10^5 and 1 x 10^6cells/mL. Do not allow the cell concentration to exceed 1 x 10^6cells/mL. Culture at 5% CO2, 37C. Feed cells once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 50% FBS and 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Suspension Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture:Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 10^5 viable cells/mL. Maintain cultures at a cell concentration between 1 x 10^5 and 1 x 10^6cells/mL. Do not allow the cell concentration to exceed 1 x 10^6cells/mL. Culture at 5% CO2, 37C. Feed cells once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 50% FBS and 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture:Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 10^5 viable cells/mL. Maintain cultures at a cell concentration between 1 x 10^5 and 1 x 10^6cells/mL. Do not allow the cell concentration to exceed 1 x 10^6cells/mL. Culture at 5% CO2, 37C. Feed cells once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 50% FBS and 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 10^5 viable cells/mL. Maintain cultures at a cell concentration between 1 x 10^5 and 1 x 10^6cells/mL. Do not allow the cell concentration to exceed 1 x 10^6cells/mL. Culture at 5% CO2, 37C. Feed cells once every 2 to 3 days. Freezing Medium:Complete culture medium supplemented with 50% FBS and 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Complete culture medium supplemented with 50% FBS and 7.5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Hepatitis B: Negative Hepatitis C: Negative
Hepatitis C: Negative
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