T11D7e2 cells

Origin:

Animals were immunized with W/Fu rat thymocytes. Spleen cells were fused with NS-1 myeloma cells.

Species:

Mouse, Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Tissue:

hybridoma: B lymphocyte

Morphology:

Lymphoblast

Properties:

Suspension

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 10^5 viable cells/mL. Maintain cultures at a cell concentration between 1 x 10^5 and 1 x 10^6cells/mL. Do not allow the cell concentration to exceed 1 x 10^6cells/mL. Culture at 5% CO2, 37C. Feed cells once every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 50% FBS and 7.5% (v/v) DMSO