L-WRN cells

Origin:

The L-WRN cells were derived by transfecting L-Wnt3A (S0011001) with an R-spondin 3 and noggin co-expressing vector and stable clones were selected in medium containing G418 and Hygromycin B. This cell line is a source for producing Wnt-3A, R-spondin 3, and noggin conditioned medium which can be used for growing various mammalian tissue stem cells.

Species:

Mouse, Mus musculus

Tissue:

Subcutaneous connective tissue; areolar and adipose

Morphology:

Fibroblast

Properties:

Adherent

Patient:

Male, C3H/An, 100 days

Medium:

AddexBio-formulated DMEM (C0003-01), or equivalent medium + 0.5 mg/mL G-418 + 0.5 mg/mL hygromycin B + 10% FBS (Wnt-3A, R-spondin 3, and noggin Conditioned Medium protocol please refer to datasheet)

Subculture:

When 80% confluent using 0.25% trypsin or trypsin/EDTA, subculture at 1:3 to 1:10; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Wnt-3A, R-spondin 3, and noggin Conditioned Medium protocol please refer to datasheet.

Freezing Medium:

Complete culture medium supplemented with 10% (v/v) DMSO