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Melanoma
Established from a B16 melanoma tumor grown in syngeneic C57BL/6 mice.
Mus musculus, mouse Tissue: Skin Morphology: Mixture of spindle-shaped and epithelial-like cells Properties: Adherent Strain: C57BL/6J Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Skin Morphology: Mixture of spindle-shaped and epithelial-like cells Properties: Adherent Strain: C57BL/6J Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Mixture of spindle-shaped and epithelial-like cells Properties: Adherent Strain: C57BL/6J Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Adherent Strain: C57BL/6J Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
C57BL/6J Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
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