Comparison

B16-F10 cells

Item no. C0012005
Manufacturer Addexbio
Amount One Frozen vial
Category
Type Tumour Cells
Specific against other
Dry ice Yes
Citations 1. Fidler IJ. Biological behavior of malignant melanoma cells correlated to their survival in vivo. Cancer Res. 1975 Jan;35(1):218-224. PMID: 1109790
ECLASS 10.1 32160890
ECLASS 11.0 32160890
UNSPSC 41106509
Available
Description

Disease:

Melanoma

Origin:

Established from a B16 melanoma tumor grown in syngeneic C57BL/6 mice.

Species:

Mus musculus, mouse

Tissue:

Skin

Morphology:

Mixture of spindle-shaped and epithelial-like cells

Properties:

Adherent

Strain:

C57BL/6J

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO

Biosafety Level:

I

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Citation Format:

If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)

Storage condition
LN2
Manufacturers Category
Cell Lines

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: One Frozen vial
Available: In stock
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