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Melanoma
Established from a B16 melanoma tumor grown in syngeneic C57BL/6 mice.
Mus musculus, mouse Tissue: Skin Morphology: Mixture of spindle-shaped and epithelial-like cells Properties: Adherent Strain: C57BL/6J Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Skin Morphology: Mixture of spindle-shaped and epithelial-like cells Properties: Adherent Strain: C57BL/6J Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Mixture of spindle-shaped and epithelial-like cells Properties: Adherent Strain: C57BL/6J Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Adherent Strain: C57BL/6J Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
C57BL/6J Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Subculture sub-confluent cultures (70-80%) at 1:10 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Yeast: Negative Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Mycoplasma: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
If use of this culture results in a scientific publication, it should be cited in the publication as: B16-F10 (AddexBio C0012005)
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
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