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Hepatoma
This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse.
Mus musculus, mouse Tissue: Liver Morphology: Epithelial Properties: Adherent Strain: C57L Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Liver Morphology: Epithelial Properties: Adherent Strain: C57L Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Epithelial Properties: Adherent Strain: C57L Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Adherent Strain: C57L Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
C57L Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
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