Hepa 1-6

Disease:
Hepatoma
Origin:
This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse.
Species:
Mus musculus, mouse
Tissue:
Liver
Morphology:
Epithelial
Properties:
Adherent
Strain:
C57L
Medium:
AddexBio-Formulated DMEM (C0003-01) + 10% FBS.
Subculture:
Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)

Cell Lines