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Hepatoma
This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse.
Mus musculus, mouse Tissue: Liver Morphology: Epithelial Properties: Adherent Strain: C57L Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Liver Morphology: Epithelial Properties: Adherent Strain: C57L Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Epithelial Properties: Adherent Strain: C57L Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Adherent Strain: C57L Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
C57L Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Subculture cells at 80% confluent at split ratio of 1:5 to 1:8 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa 1-6 (AddexBio C0015005)
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
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