LL/2

Disease:
Lewis lung carcinoma
Origin:
Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.
Species:
Mus musculus, mouse
Tissue:
Lung
Morphology:
Rounded-loosely attached or floating
Properties:
Mixed, adherent and suspension
Strain:
C57BL
Medium:
AddexBio-Formulated DMEM (C0003-01) + 10% FBS.
Subculture:
Subculture cells at 80% confluent at split ratio of 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: LL/2 (AddexBio C0016030)

Cell Lines