LL/2

Disease:

Lewis lung carcinoma

Origin:

Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.

Species:

Mus musculus, mouse

Tissue:

Lung

Morphology:

Rounded-loosely attached or floating

Properties:

Mixed, adherent and suspension

Strain:

C57BL

Medium:

AddexBio-Formulated DMEM (C0003-01) + 10% FBS.

Subculture:

Subculture cells at 80% confluent at split ratio of 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO