H2122 cells

Disease:

Stage 4, adenocarcinoma; non-small cell lung cancer.

Origin:

The line was established in January 1989 from pleural effusion metastasis of lung.

Species:

Homo sapiens, human

Tissue:

Lung; derived from metastatic site: pleural effusion

Morphology:

Lymphoblast

Properties:

These cells show a mixed morphology of rounded cells and epithelial cells. This cell line produces moderate to large, viable, loose, grape-like clusters that either loosely adhere to the flask or remain in suspension.

Patient:

Caucasian, female, 46 years of age, the patient was a smoker at 30 pack year.

Medium:

AddexBio-formulated RPMI-1640 Medium (C0004-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:3 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO