U-251MG cells

Disease:
Human glioblastoma astrocytoma
Origin:
Derived from a malignant glioblastoma tumor by explant technique.
Species:
Homo sapiens, human
Tissue:
Brain
Morphology:
Pleomorphic/astrocytoid
Properties:
Adherent
Cytogenic data:
2n = 46
Medium:
AddexBio-formulated EMEM Medium (C0005-01) + 10% FBS
Subculture:
Subculture sub-confluent cultures (70-80%) at 1:3 to 1:6 ratio, i.e. seeding at 2-4x10, 000 cells/cm^2 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 11, 12
D13S317: 10, 11
D16S539: 12
D5S818:11, 12
D7S820: 10, 12
THO1: 9.3
TPOX: 8
vWA: 16, 18
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)

Cell Lines