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Human glioblastoma astrocytoma
Derived from a malignant glioblastoma tumor by explant technique.
Homo sapiens, human Tissue: Brain Morphology: Pleomorphic/astrocytoid Properties: Adherent Cytogenic data: 2n = 46 Medium: AddexBio-formulated EMEM Medium (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:6 ratio, i.e. seeding at 2-4x10, 000 cells/cm^2 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
Brain Morphology: Pleomorphic/astrocytoid Properties: Adherent Cytogenic data: 2n = 46 Medium: AddexBio-formulated EMEM Medium (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:6 ratio, i.e. seeding at 2-4x10, 000 cells/cm^2 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
Pleomorphic/astrocytoid Properties: Adherent Cytogenic data: 2n = 46 Medium: AddexBio-formulated EMEM Medium (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:6 ratio, i.e. seeding at 2-4x10, 000 cells/cm^2 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
Adherent Cytogenic data: 2n = 46 Medium: AddexBio-formulated EMEM Medium (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:6 ratio, i.e. seeding at 2-4x10, 000 cells/cm^2 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
2n = 46 Medium: AddexBio-formulated EMEM Medium (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:6 ratio, i.e. seeding at 2-4x10, 000 cells/cm^2 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
AddexBio-formulated EMEM Medium (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:6 ratio, i.e. seeding at 2-4x10, 000 cells/cm^2 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
Subculture sub-confluent cultures (70-80%) at 1:3 to 1:6 ratio, i.e. seeding at 2-4x10, 000 cells/cm^2 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
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