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Sarcoma
This cell line was derived from metastatic site: ascites.
Mus musculus, mouse Tissue: Sarcoma Morphology: Suspension Properties: Suspension Strain: Swiss Webster Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/mL and maintain between 1 X 10^5 and 1 X 10^6 cells/mL. Culture at 5% CO2, 37C. Change medium every 2-3 days. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
Sarcoma Morphology: Suspension Properties: Suspension Strain: Swiss Webster Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/mL and maintain between 1 X 10^5 and 1 X 10^6 cells/mL. Culture at 5% CO2, 37C. Change medium every 2-3 days. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
Suspension Properties: Suspension Strain: Swiss Webster Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/mL and maintain between 1 X 10^5 and 1 X 10^6 cells/mL. Culture at 5% CO2, 37C. Change medium every 2-3 days. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
Suspension Strain: Swiss Webster Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/mL and maintain between 1 X 10^5 and 1 X 10^6 cells/mL. Culture at 5% CO2, 37C. Change medium every 2-3 days. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
Swiss Webster Medium: AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/mL and maintain between 1 X 10^5 and 1 X 10^6 cells/mL. Culture at 5% CO2, 37C. Change medium every 2-3 days. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
AddexBio-formulated DMEM (C0003-01) + 10% FBS Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/mL and maintain between 1 X 10^5 and 1 X 10^6 cells/mL. Culture at 5% CO2, 37C. Change medium every 2-3 days. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10^5 cells/mL and maintain between 1 X 10^5 and 1 X 10^6 cells/mL. Culture at 5% CO2, 37C. Change medium every 2-3 days. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
If use of this culture results in a scientific publication, it should be cited in the publication as: Sarcoma 180 (AddexBio C0035002)
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