INS-1 832/13 cells

Disease:

Insulinoma

Origin:

INS-1 832/13 is a derivative of INS-1 (C0018007) cells originally established from an x-ray induced insulinoma in rat. The INS-1 832/13 cell line is a subclone of INS-1 that was stably transfected with a CMV promoter-human ins expression plasmid carrying a geneticin (G418)-resistance marker for selection.

Species:

Rattus Norvegicus

Tissue:

Pancreas

Morphology:

Beta cells

Properties:

Adherent

Medium:

AddexBio-Optimized RPMI-1640 Medium(C0004-02) + 10% FBS + 0.05 mM 2-mercaptoethanol

Subculture:

Subculture sub-confluent cultures (85%) at 1:5 to 1:6 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 10% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO