INS-1 832/13 cells

Disease:
Insulinoma
Origin:
INS-1 832/13 is a derivative of INS-1 (C0018007) cells originally established from an x-ray induced insulinoma in rat. The INS-1 832/13 cell line is a subclone of INS-1 that was stably transfected with a CMV promoter-human ins expression plasmid carrying a geneticin (G418)-resistance marker for selection.
Species:
Rattus Norvegicus
Tissue:
Pancreas
Morphology:
Beta cells
Properties:
Adherent
Medium:
AddexBio-Optimized RPMI-1640 Medium(C0004-02) + 10% FBS + 0.05 mM 2-mercaptoethanol
Subculture:
Subculture sub-confluent cultures (85%) at 1:5 to 1:6 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 10% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
II
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
HPV: Negative
EBV: Negative
CMV: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: INS-1 832/13 (AddexBio C0018024)

Cell Lines