QCRL-3

Disease:

Carcinoma

Origin:

Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.

Species:

Mus musculus (B cell), Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Tissue:

Spleen

Morphology:

Lymphoblast, hybridoma: B lymphocyte

Properties:

Suspension

Strain:

BALB/c (B cell); BALB/c (myeloma)

Medium:

AddexBio-Formulated DMEM (C0003-01) + 10% FBS.

Subculture:

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10^5 viable cells/ml. Interval: Maintain cell density between 1 X 10^5 and 1 X 10^6 viable cells/ml. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO