QCRL-3

Disease:
Carcinoma
Origin:
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
Species:
Mus musculus (B cell), Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue:
Spleen
Morphology:
Lymphoblast, hybridoma: B lymphocyte
Properties:
Suspension
Strain:
BALB/c (B cell); BALB/c (myeloma)
Medium:
AddexBio-Formulated DMEM (C0003-01) + 10% FBS.
Subculture:
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10^5 viable cells/ml. Interval: Maintain cell density between 1 X 10^5 and 1 X 10^6 viable cells/ml. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)

Cell Lines