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Carcinoma
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
Mus musculus (B cell), Mus musculus (myeloma), mouse (B cell); mouse (myeloma) Tissue: Spleen Morphology: Lymphoblast, hybridoma: B lymphocyte Properties: Suspension Strain: BALB/c (B cell); BALB/c (myeloma) Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10^5 viable cells/ml. Interval: Maintain cell density between 1 X 10^5 and 1 X 10^6 viable cells/ml. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
Spleen Morphology: Lymphoblast, hybridoma: B lymphocyte Properties: Suspension Strain: BALB/c (B cell); BALB/c (myeloma) Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10^5 viable cells/ml. Interval: Maintain cell density between 1 X 10^5 and 1 X 10^6 viable cells/ml. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
Lymphoblast, hybridoma: B lymphocyte Properties: Suspension Strain: BALB/c (B cell); BALB/c (myeloma) Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10^5 viable cells/ml. Interval: Maintain cell density between 1 X 10^5 and 1 X 10^6 viable cells/ml. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
Suspension Strain: BALB/c (B cell); BALB/c (myeloma) Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10^5 viable cells/ml. Interval: Maintain cell density between 1 X 10^5 and 1 X 10^6 viable cells/ml. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
BALB/c (B cell); BALB/c (myeloma) Medium: AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10^5 viable cells/ml. Interval: Maintain cell density between 1 X 10^5 and 1 X 10^6 viable cells/ml. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
AddexBio-Formulated DMEM (C0003-01) + 10% FBS. Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10^5 viable cells/ml. Interval: Maintain cell density between 1 X 10^5 and 1 X 10^6 viable cells/ml. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10^5 viable cells/ml. Interval: Maintain cell density between 1 X 10^5 and 1 X 10^6 viable cells/ml. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
If use of this culture results in a scientific publication, it should be cited in the publication as: QCRL-3 (AddexBio C0033010)
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
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