HEL 299 cells

Disease:

Normal

Origin:

Derived from embryonic lung tissue of a male in 1965. It is one of the few human diploid lines to have type A glucose-6-phosphate dehydrogenase. The capacity of this cell line to propagate in culture is limited, and they will eventually senesce. Cells from the current distribution stocks are capable of at least 15 population doublings before the onset of senescence.

Species:

Homo sapiens, human

Tissue:

Embryo lung

Morphology:

Fibroblast

Properties:

Adherent

Cytogenic data:

normal human male; diploid; stable. 2n = 46

Patient:

Black, male, fetus.

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO