HEL 299 cells

Disease:
Normal
Origin:
Derived from embryonic lung tissue of a male in 1965. It is one of the few human diploid lines to have type A glucose-6-phosphate dehydrogenase. The capacity of this cell line to propagate in culture is limited, and they will eventually senesce. Cells from the current distribution stocks are capable of at least 15 population doublings before the onset of senescence.
Species:
Homo sapiens, human
Tissue:
Embryo lung
Morphology:
Fibroblast
Properties:
Adherent
Cytogenic data:
normal human male; diploid; stable. 2n = 46
Patient:
Black, male, fetus.
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 7, 10
D13S317: 11, 12
D16S539: 10, 11
D5S818:11, 13
D7S820: 8, 11
THO1:7
TPOX: 8, 12
vWA: 16
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)

Cell Lines