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Normal
Derived from embryonic lung tissue of a male in 1965. It is one of the few human diploid lines to have type A glucose-6-phosphate dehydrogenase. The capacity of this cell line to propagate in culture is limited, and they will eventually senesce. Cells from the current distribution stocks are capable of at least 15 population doublings before the onset of senescence.
Homo sapiens, human Tissue: Embryo lung Morphology: Fibroblast Properties: Adherent Cytogenic data: normal human male; diploid; stable. 2n = 46 Patient: Black, male, fetus. Medium: AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
Embryo lung Morphology: Fibroblast Properties: Adherent Cytogenic data: normal human male; diploid; stable. 2n = 46 Patient: Black, male, fetus. Medium: AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
Fibroblast Properties: Adherent Cytogenic data: normal human male; diploid; stable. 2n = 46 Patient: Black, male, fetus. Medium: AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
Adherent Cytogenic data: normal human male; diploid; stable. 2n = 46 Patient: Black, male, fetus. Medium: AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
normal human male; diploid; stable. 2n = 46 Patient: Black, male, fetus. Medium: AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
Black, male, fetus. Medium: AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
AddexBio-formulated EMEM (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
Amelogenin: X, Y CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
CSF1PO: 7, 10 D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
D13S317: 11, 12 D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
D16S539: 10, 11 D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
D5S818:11, 13 D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
D7S820: 8, 11 THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
THO1:7 TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
TPOX: 8, 12 vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
vWA: 16 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
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