Comparison

HEL 299 cells

Item no. T0016051
Manufacturer Addexbio
Amount One Frozen vial
Category
Type Tumour Cells
Specific against other
Dry ice Yes
Citations 1. Peterson WD Jr, Stulberg CS, Swanborg NK, Robinson AR. Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc Soc Exp Biol Med. 1968 Jul;128(3):772-776. PMID: 5668122
ECLASS 10.1 32160890
ECLASS 11.0 32160890
UNSPSC 41106509
Available
Description

Disease:

Normal

Origin:

Derived from embryonic lung tissue of a male in 1965. It is one of the few human diploid lines to have type A glucose-6-phosphate dehydrogenase. The capacity of this cell line to propagate in culture is limited, and they will eventually senesce. Cells from the current distribution stocks are capable of at least 15 population doublings before the onset of senescence.

Species:

Homo sapiens, human

Tissue:

Embryo lung

Morphology:

Fibroblast

Properties:

Adherent

Cytogenic data:

normal human male; diploid; stable. 2n = 46

Patient:

Black, male, fetus.

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO

Biosafety Level:

I

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

HIV: Negative

Hepatitis B: Negative

DNA Profile (STR):

Amelogenin: X, Y

CSF1PO: 7, 10

D13S317: 11, 12

D16S539: 10, 11

D5S818:11, 13

D7S820: 8, 11

THO1:7

TPOX: 8, 12

vWA: 16

Citation Format:

If use of this culture results in a scientific publication, it should be cited in the publication as: HEL 299 (AddexBio T0016051)

Storage condition
LN2
Manufacturers Category
Cell Lines

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: One Frozen vial
Available: In stock
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