3D4/2 cells

Disease:

Normal macrophage; SV40 Immortalized

Origin:

The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid.

Species:

Sus scrofa, pig

Tissue:

Lung

Morphology:

Macrophage

Properties:

Adherent

Cytogenic data:

Not available

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS

Subculture:

When reaching 2 x 10^5 cells/cm^2 using 0.25% trypsin or trypsin/EDTA, subculture at 1:6 to 1:8; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Start culture at 5 x 10^3 cells/cm2.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO