3D4/2 cells

Disease:
Normal macrophage; SV40 Immortalized
Origin:
The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid.
Species:
Sus scrofa, pig
Tissue:
Lung
Morphology:
Macrophage
Properties:
Adherent
Cytogenic data:
Not available
Medium:
AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS
Subculture:
When reaching 2 x 10^5 cells/cm^2 using 0.25% trypsin or trypsin/EDTA, subculture at 1:6 to 1:8; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Start culture at 5 x 10^3 cells/cm2.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
II
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
If use of this culture results in a scientific publication, it should be cited in the publication as: 3D4/2 (AddexBio T0016001)
Related cells by Organ: A427 cells
A549 cells
Calu3 cells
H1650 cells
H1703 cells
H1975 cells
H226 cells
H23 cells
H460 cells
H520 cells
HCC4006 cells
SW1573 cells
3D4/2 cells
BEAS-2B cells
MRC-5 cells
Mv 1 Lu cells
RLE-6TN cells

Cell Lines