RLE-6TN cells

Disease:
Normal (Immortalized)
Origin:
Expression of the SV40-T antigen was negative by nuclear immunostaining and by PCR, indicating these cells were derived by a spontaneous immortalization. The RLE-6TN (rat lung epithelial-T-antigen negative) cell line was derived from alveolar Type II cells isolated from a 56 day old male F344 rat using airway perfusion with a pronase solution.
Species:
Rattus norvegicus, rat
Tissue:
Lung
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Cells are reported to remain near diploid and karyotypically stable from passage 19-70 with 50% or more of the cells containing 42 chromosomes. At passage 37, there was a translocation between chromosome 1 and 15 which results in trisomy of the q arm of chromosome 1.
Strain:
male The RLE-6TN (rat lung epithelial-T-antigen negative) cell line was derived from alveolar Type II cells isolated from a 56 day old male Fischer 344 (F344) rat using airway perfusion with a pronase solution.
Medium:
Ham's F12 medium with 2 mM L-glutamine supplemented with 0.01 mg/ml bovine pituitary extract, 0.005 mg/ml insulin, 2.5 ng/ml insulin-like growth factor, 0.00125 mg/ml transferrin, and 2.5 ng/ml EGF, 90% + 10% FBS
Subculture:
Split the cells at 80% confluent culture at 1:5. Culture at 5% CO2, 37C.
Freezing Medium:
Complete culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
II
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: RLE-6TN (AddexBio C0016014)
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RLE-6TN cells

Cell Lines