RLE-6TN cells

Disease:

Normal (Immortalized)

Origin:

Expression of the SV40-T antigen was negative by nuclear immunostaining and by PCR, indicating these cells were derived by a spontaneous immortalization. The RLE-6TN (rat lung epithelial-T-antigen negative) cell line was derived from alveolar Type II cells isolated from a 56 day old male F344 rat using airway perfusion with a pronase solution.

Species:

Rattus norvegicus, rat

Tissue:

Lung

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

Cells are reported to remain near diploid and karyotypically stable from passage 19-70 with 50% or more of the cells containing 42 chromosomes. At passage 37, there was a translocation between chromosome 1 and 15 which results in trisomy of the q arm of chromosome 1.

Strain:

male The RLE-6TN (rat lung epithelial-T-antigen negative) cell line was derived from alveolar Type II cells isolated from a 56 day old male Fischer 344 (F344) rat using airway perfusion with a pronase solution.

Medium:

Ham's F12 medium with 2 mM L-glutamine supplemented with 0.01 mg/ml bovine pituitary extract, 0.005 mg/ml insulin, 2.5 ng/ml insulin-like growth factor, 0.00125 mg/ml transferrin, and 2.5 ng/ml EGF, 90% + 10% FBS

Subculture:

Split the cells at 80% confluent culture at 1:5. Culture at 5% CO2, 37C.

Freezing Medium:

Complete culture medium supplemented with 7.5% (v/v) DMSO