Active Recombinant human Growth Hormone

C1025H1570N280O306S7

0.77

Endotoxin Level : < 0.04 EU / ug protein (LAL method)

GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation, an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.

Recombinant human GH is lyophilized from 10mM Phosphate Potasium buffer pH 7, 6 and 0.05M NaCl.

Functional studies, Cell culture, Western Blot, Dermocosmetics (rh GH stimulates fibroblast cell proliferation, kerotinocytes migration (HaCaT cells) and induces whitening activity ).

2. Effect of rH GH on kerotinocytes migration (HaCaT cells). - Cell migration after 24h of treatment with different concentrations of rh GH, scale bar 100 px, A: Untreated cells, B: Positive control Growth factor cocktail 10% , C: 1 ng/ml rH GH, D: 5ng/ml rH GH.

4. Whitening activity of rh GH on in vitro reconstructed tissues of pigmented skin. After treatment tissues were evaluated by histology analysis (Haematoxylin – Eosin staining), cell viability and melanin quantification. - Melanin quantification in RHPE treated tissues. A: Untreated cells, B: Positive control (2% Ascorbic acid) Cell viability 93% , rh GH (5 ng/ml) Cell viability 96%.

Centrifuge vial before opening. Lyophilized protein should be reconstituted in water to a concentration of 50 ng/ul. Optimal concentration should be determined for specific application and cell lines.

Room temperature