omega-Conotoxin MVIIC- Biotin

Non Antibodies

Proteins

Biotin. The extent of labeling is 1-2 dye molecules of biotin per ω-Conotoxin MVIIC molecule, biotinylated through amines.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

~ 3257 Da

Biotinylated ω-Conotoxin MVIIC for Sensitive Detection of Presynaptic CaV (N- and P/Q-Type) Channels

Centrifuge the vial (10, 000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (5 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

12352202

Disulfide bonds between: Cys1-Cys16, Cys8-Cys20 and Cys15-Cys26Cys26 - C-terminal amidation Biotin

ω-Conotoxin MVIIC blocks P/Q- and N-type CaV channels1. ω-Conotoxin MVIIC is also reported to block nicotinic receptors heterologously expressed in Xenopus oocytes with an IC50 of 1.3 μM2.

Yes