omega-Conotoxin MVIIC-ATTO Fluor 647N

Non Antibodies

Proteins

ATTO-647N. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. Maximum absorption 646 nm; maximum fluorescence 664 nm. The fluorescence is excited most efficiently in the range 625 - 660 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm. It can be used in flow cytometry (FACS) using the red (637 nm) laser. The extent of labeling is one molecule of dye per one ω-Conotoxin MVIIC molecule.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Avoid exposure to light.

3376 Da

A Fluorescent Conjugate Marker for Sensitive Detection of Presynaptic CaV (N- and P/Q-Type) Channels

Centrifuge the vial (10, 000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 ml conical vial. The product is soluble in pure water to high-micromolar concentrations (5 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.

12352202

Disulfide bonds between: Cys1-Cys16, Cys8-Cys20 and Cys15-Cys26Cys26 - C-terminal amidation ATTO-Fluor 647N

ω-Conotoxin MVIIC blocks P/Q- and N-type CaV channels1. ω-Conotoxin MVIIC is also reported to block nicotinic receptors heterologously expressed in Xenopus oocytes with an IC50 of 1.3 μM2.

Yes