GsMTx-4-Biotin

ArtNr	ALO-STG-100-B-5ug
Hersteller	Alomone
Menge	5 ug
Quantity options	10 ug 10 x 10 ug 2 x 10 ug 5 ug 5 x 10 ug
Kategorie	Peptides & Proteins Flow Cytometry Immunofluorescence Native Proteins
Typ	Proteins Native
Format	Lyophilized
Applikationen	FC, IF
Specific against	other
Konjugat/Tag	Biotin
Formula	Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
Sequence	GCLEFWWKCNPNDDKCCRPKLKCSKLFKLCNFSF-NH2
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Versandbedingung	Raumtemperatur
Lieferbar
Manufacturer - Type	Non Antibodies
Manufacturer - Applications	Electrophysiology, Live cell imaging, Immunofluorescence, Fluorescence staining, Indirect flow cytometry
Manufacturer - Category	Proteins
Manufacturer - Targets	NaV1.7 channels, TRPC1, TRPC6, Piezo1
Manufacturer - Conjugate / Tag	LC-Biotin. The extent of labeling is 1 molecule of biotin per molecule of GsMTx-4.
Country of Origin	Israel
Shipping Temperature	The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.
Storage Conditions	Storage as Solution: Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. - Storage before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. - Storage after Reconstitution: Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.
Molecular Weight	4435 Da
Manufacturer - Format	Lyophilized
Short description	Biotinylated GsMTx-4 for Sensitive Detection of NaV1.7, TRPC1, TRPC6 and Piezo1 Channels
Description	M-TRTx-Gr1a, M-theraphotoxin-Gr1a, GsMTx4 - Biotinylated GsMTx-4 for Sensitive Detection of NaV1.7, TRPC1, TRPC6 and Piezo1 Channels
Reconstitution	Centrifuge the vial (10, 000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (5 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
PH	7, 4
UNSPSC	12352202
Origin	Grammostola rosea (Chilean rose) tarantula
Modifications	Disulfide bonds between: Cys2-Cys17, Cys9-Cys23, and Cys16-Cys30 Phe34 - C-terminal amidationLC-Biotin
Effective Concentration	1.5 µM – 5 µM
Activity	GsMTx-4 is an inhibitor of Na+ voltage-gated channels3 and cation-selective mechanosensitive channels1, 2.
Solubility	Centrifuge the vial before adding solvent (10, 000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. Soluble in pure water at high-micromolar concentrations (50 µM - 1 mM). For long-term storage in solution, it is recommended to prepare a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations at 10, 000 x g for 5 minutes before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity
Sterile endotoxin free	no
Bioassay tested	yes
Scientific Background	GsMTx-4 is a 34 amino acid peptidyl toxin originally isolated from the Grammostola rosea (Chilean rose) tarantula venom and belongs to the huwentoxin-1 family1. This toxin inhibits different channels and in addition has antimicrobial activity. It blocks cation-selective mechanosensitive ion channels (strech-activated channels, SACs), without having an effect on whole-cell voltage-sensitive currents1. In addition, it inhibits atrial fibrillation2 as well as the membrane motor of outer hair cells3 at low doses. A medium toxicity on a large spectra of voltage-gated Na+ channels, namely NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.5, NaV1.6 and NaV1.7 was reported. GsMTx-4 also inhibits K+ channels KV11.1 and KV11.2, whereas it does not inhibit K+ channels KV1.1 (IC50 > 85 µM), KV1.4 (IC50 > 85 µM) and KV11.3 (IC50 = 53 µM)4. GsMTx-4 was also found to inhibit both TRPC1 and TRPC6 channels5, 6, as well as Piezo1, the mechanosensitive channel7. Antimicrobial activity is shown against several Gram-positive and Gram-negative bacteria as well8.

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ALO-STG-100-B-5ug-safety-datasheet.pdf