R-Spondin-2, Human, Recombinant

The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization and resulting accumulation of the intracellular signaling protein, beta-catenin. This activity effectively activates and increases the subsequent nuclear signaling of beta-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/ beta-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations. Recombinant human R-Spondin-2 is a 24.4 kDa protein consisting of 212 amino acid residues. Due to glycosylation, R-Spondin-2 migrates at an apparent molecular weight of approximately 30.0 kDa by SDS PAGE analysis under reducing conditions.

Roof plate-specific, spondin-2, RSPO2

CHO cells

ASYVSNPICK GCLSCSKDNG CSRCQQKLFF FLRREGMRQY GECLHSCPSG YYGHRAPDMN RCARCRIENC DSCFSKDFCT KCKVGFYLHR GRCFDECPDG FAPLEETMEC VEGCEVGHWS EWGTCSRNNR TCGFKWGLET RTRQIVKKPV KDTILCPTIA ESRRCKMTMR HCPGGKRTPK AKEKRNKKKK RKLIERAQEQ HSVFLATDRA NQ

R-Spondin-2 enhances BMP-2 mediated differentiation of MC3T3-E1 cells.

Verified by N-terminal and Mass Spectrometry analyses (when applicable).