SensoLyte® Green Protease Assay Kit Fluorimetric - 1 kit

Assay Kits

4 °C

The SensoLyte Green Protease Assay Kit is widely used for detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity (Ex/Em=488/520 nm). The SensoLyte protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases. The kit contains: - Fluorescent labeled casein with high ratio of dye/protein (pH-insensitive) - Trypsin (as positive control) - Assay buffer - A detailed protocol

Cell Biology & Protein Analysis / 0

520

Fluorescent

41105331