SensoLyte® Green Protease Assay Kit Fluorimetric - 1 kit

Protease activity

Protease

Store component A and B at -20°C. Protect component A from light and moisture. Component C can be stored at room temperature for convenience.

The SensoLyte® Green Protease Assay Kit is widely used for detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity (Ex/Em=488/520 nm). The SensoLyte® protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases.

488

Fluorescent

Research use

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