SensoLyte® Red Protease Assay Kit Fluorimetric - 1 kit

Protease activity

Protease

Store component A and B at -20°C. Protect component A from light and moisture. Component C can be stored at room temperature for convenience.

The SensoLyte® Red Protease Assay Kit is widely used for continuous detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Compared to the SensoLyte® Green Protease Assay Kit, this kit contains a red fluorescent casein derivative. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly red fluorescent dye-labeled peptides (Ex/Em=546/575 nm). The increase in fluorescence intensity is directly proportional to protease activity. The SensoLyte® protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases. The kit includes: - Fluorescently labeled casein with high ratio of dye/protein - Trypsin (as positive control) - Assay buffer - A detailed protocol

546

Fluorescent

Research use

5400535133054