AnaTag™ 5 Kit - 1 kit

Protein labeling kits

Assay Kits - AnaTag / Assay Kits - AnaTag / Labeling & detection / Fluorescent Dye

Store component A at -20°C. Store all other components at 4°C. Protect component A from light and moisture.

Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. 5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivity to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels. 5-FITC is more widely used than 6-FITC isomer for protein labeling. FITC-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all the essential components for performing the conjugation reaction and for purifying the FITC-protein conjugates. The kit is good for three conjugation reactions with each reaction, labeling up to 200 µg IgG.
Note: AnaTag kits are not intended for peptide labeling.

494

Fluorescent

No

41105331