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AnaTag™ 5 Kit - 1 kit

AnaTag™ 5 Kit - 1 kit

Item no.	AS-72060
Manufacturer	AnaSpec
Amount	1 kit
Category	Reagents & Chemicals Staining & Dyes Protein Stains
Type	Kit
Specific against	other
Dry ice	Yes
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Alias	Protein labeling kit
Shipping Condition	Dry ice
Available
Manufacturer - Type	Protein labeling kits
Manufacturer - Applications	Labeling
Manufacturer - Category	Assay Kits - AnaTag / Assay Kits - AnaTag / Labeling & detection / Fluorescent Dye
Shipping Temperature	4 °C
Storage Conditions	Store component A at -20°C. Store all other components at 4°C. Protect component A from light and moisture.
Manufacturer - Research Area	Peptide & Protein Analysis / Protein Labeling Kits
Description	Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents, probably due to the low cost of the material. 5-FITC and 6-FITC have very similar absorption and fluorescence spectra. However, the isomers may differ in their binding and reactivity to proteins, and the conjugates may elute under different chromatographic conditions or migrate differently in electrophoretic gels. 5-FITC is more widely used than 6-FITC isomer for protein labeling. FITC-protein conjugates are suitable for immunofluorescent staining, in situ hybridization, flow cytometry and other biological applications. The kit has all the essential components for performing the conjugation reaction and for purifying the FITC-protein conjugates. The kit is good for three conjugation reactions with each reaction, labeling up to 200 µg IgG. Note: AnaTag kits are not intended for peptide labeling.
Kits Components	Component A: 5-FITC Amino-reactive dye: 3 vials Component B: Reaction buffer for pH adjustment of the conjugation reaction: 0.5 mL Component C: Spin column to purify dye-protein conjugate: 3 pre-packed columns Component D: DMSO solvent for preparing dye stock solution: 150 µL Component E: Elution buffer for eluting dye-protein conjugate: 20 mL Component F: Wash tube to hold buffer for Spin column: 3 tubes Component G: Collect tube to collect dye-protein conjugate: 3 tubes
Absorbtion (nm)	494
Emission (nm)	519
Detection Method	Fluorescent
Feature	Green (500 -550 nm)
Post Synthesis Coupling	No
Usage	Research use
UNSPSC	41105331
GTIN	5400535132026

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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AS-72060-safety-datasheet.pdf