Vergleich

AnaTag™ R - PE Labeling Kit - 1 kit

ArtNr AS-72113
Hersteller AnaSpec
Menge 1 kit
Kategorie
Typ Kit
Specific against other
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Protein labeling kits
Manufacturer - Applications
Labeling
Manufacturer - Category
Labeling & detection
Shipping Temperature
4 °C
Storage Conditions
Store all components at 4°C. Protect component A from light and do not freeze.Components B and H may be frozen for best performance.
Description
The AnaTag R-PE Labeling Kit is optimized for use in the conjugation of R-Phycoerythrin (R-PE) to antibodies. R-PE, a fluorescent protein from phycobiliprotein family, has broad absorption bands with peaks at 565 nm (eM =1.96 X 106 M-1cm-1), 498 (eM =1.53 X 106 M-1cm-1), and 539 nm (eM =1.62 X 106 M-1cm-1); it therefore can be excited with versatile excitation sources. Its maximal emission can be detected at 578 nm. The broad excitation spectrum also provides the advantage of multi-color immunofluorescent staining or cell sorting. R-PE and the closely related B-PE are the most intensely fluorescent phycobiliproteins having orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores. The AnaTagTM R-PE Labeling Kit contains SH-reactive R-PE. SMCC modified R-PE reacts with the thiol groups of target antibody without the need for additional activation, thus simplifying the conjugation protocol. The amount of R-PE supplied in this kit is sufficient for labeling up to 1 mg of antibody. The kit provides all reagents, purification columns and a detailed step-by-step protocol. Native and SMCC-activated R-PE are also available as separate products.
Product Group
NC1ZZZ
Research Area
Peptide & Protein Analysis / 0
Absorbtion (nm)
565
Emission (nm)
578
GTIN
5400535131562
Detection Method
Fluorescent
Usage
Research use
UNSPSC
41105331

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 kit
Lieferbar: In stock
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