ArtNr |
CLS-330364 |
Hersteller |
CLS Cell Lines Service
|
Menge |
1 cell culture flask |
Kategorie |
|
Typ |
Cell line |
Specific against |
other |
Dry ice |
Yes
|
ECLASS 10.1 |
42040401 |
ECLASS 11.0 |
42040401 |
UNSPSC |
41106509 |
Alias |
U2OS, U-2OS, U-2-OS, U2-OS, U20-S, U20S, 2T |
Lieferbar |
|
Growth properties |
Monolayer, adherent |
Age |
15 years |
Gender |
Female |
Ethnicity |
Caucasian |
Morphology |
Epithelial-like |
Biosafety Level |
1 |
Culture Medium |
DMEM:Ham' s F12 |
Medium Supplements |
5% FBS, 2 mM L-glutamine |
Subculturing |
Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. |
Fluid Renewal |
2 - 3 times per week |
Freeze Medium |
CM-ACF (CLS catalog number 800650) |
Split Ratio |
A ratio of 1:3 to 1:6 is recommended |
Seeding Density |
1 x 10^4 cells/cm^2 |
Antigen Expression |
Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) |
Products |
Osteosarcoma derived growth factor (ODGF) |
Karyotype |
(P11-46) hypodiploid to near tetraploid; (P111-118) modal numbers 34 to 37 and 64 to 67 with abnormalities including dicentrics, breaks, rings, and pulverizations plus acrocentric subtelocentric and minute markers |
Receptors Expressed |
Insulin-like growth factor I (IGF-I); insulin-like growth factor II (IGF-I); Osteosarcoma derived growth factor (ODGF) |
Isoenzymes |
PGM3, 1; PGM1, 2; ES-D, 1; AK-1, 1; GLO-1, 2; G6PD, B; Phenotype Frequency Product: 0.0082 |
Manufacturer - Citation |
U-2 OS (CLS catalog number 300364) |
CSF1PO |
12, 13 |
D13S317 |
13 |
D16S539 |
11, 12 |
D5S818 |
8, 11 |
D7S820 |
11, 12 |
TH01 |
6, 9.3 |
TPOX |
11, 12 |
vWA |
14, 18 |
D3S1358 |
16 |
D21S11 |
31 |
D18S51 |
12, 14 |
D8S1179 |
12, 14 |
FGA |
20 |
D2S1338 |
20, 24 |
D19S433 |
15 |
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