SensoLyte® 520 Granzyme A Activity Assay Kit, Fluorimetric - 1 kit

Assay Kits

4 °C

Granzyme A is the most abundant serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A enters pathogen-containing and tumor cells by the perforin pathway and induces caspase-independent apoptosis. Thus, cells that are resistant to the caspases or Granzyme B (a chief apoptotic mediator) might be sensitive to Granzyme A. Recent studies showed that Granzyme A activates a novel programmed cell death pathway that begins in the mitochondrion. Granzyme A also targets several important nuclear proteins for degradation including histones and DNA damage repair proteins. Granzyme A has proinflammatory activity inducing certain cytokines secretion. SensoLyte520 Granzyme A Assay Kit provides a convenient assay for screening of enzyme inhibitors or for detecting the activity of enzyme using a novel internally quenched 5-FAM/QXL FRET substrate. Upon cleavage by granzyme A, the FRET substrate will be separated into two fragments resulting in release of 5-FAM fluorescence which can be monitored at excitation /emission= 490 nm/520 nm. The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. This kit can be used to detect the activity of Granzyme A enzyme in biological samples and purified enzyme preparations.The kit contains: QXL 520/ 5-FAM-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) Assay buffer Inhibitor Fluorescence reference standard for calibration A detailed protocol Kit size: 100 assays (96-well plate)

Cancer & Apoptosis / 0

520

Fluorescent

41105331