SensoLyte® 520 Granzyme B Activity Assay Kit, Fluorimetric - 1 kit

Enzyme activity; Screening; Protease activity

Granzyme B

Dry Ice

Granzyme B is a serine protease mostly secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. It is one of the major mediators of caspase-dependent apoptosis. Recent studies showed that Granzyme B can also be secreted by various non-cytotoxic cells and involved in degradation of extracellular matrix proteins thereby inducing inflammation and impairing wound healing. Thus, if intracellular activity of Granzyme B is mainly related to apoptosis, its extracellular activity has been linked to roles in arthritis, vascular pathologies, and other diseases. The SensoLyte 520 Granzyme B Assay Kit provides a convenient assay for screening of enzyme inhibitors or for continuous assay of Granzyme B activity using a novel internally quenched 5-FAM/QXLFRET substrate. Upon cleavage by Granzyme B, the FRET substrate will be separated into two fragments resulting in release of 5-FAM fluorescence which can be monitored at excitation /emission= 490 nm/520 nm. The kit can be used to detect the activity of Granzyme B enzyme in biological samples and purified enzyme preparations. This assay can detect as low as 0.1 ng/mL active granzyme B. The kit contains: QXL 520/ 5-FAM-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage) Assay buffer Recombinant Human Granzyme B Inhibitor Fluorescence reference standard for calibration A detailed protocol Kit size: 100 assays (96-well plate)

Cancer & Apoptosis / 0

520

Green

Research use